how to draw 3d contour lines
How to Excerpt a 3D Object in Imaris Using the Profile Surface | Tutorial
Using the Contour Surface
The Contour Surface allows you to extract a 3D object past manually drawing the object contours on 2D slices while focusing on specific details. You can utilize the Contour Surface method whenever automatic surface sectionalization does not yield individual structures in a satisfying way (e.thou. touching cells in confocal microscopy, complex tissue recorded past transmission microscopy).
To start the Contour surface cosmos, you have to cancel the Surface creation wizard. You lot can do that either by clicking on the Cancel button or by selecting the Skip automatic creation, go to manual editing push.
The creation wizard is halted and Draw Tab is automatically opened. In the viewing area, a drawing plane is displayed. To provide an unobstructed slice view, switch off the Volume rendering.
In order to become the required view, it may be necessary to re-piece the prototype information in a different orientation. Under the Board tab, orientation options allow you to piece data in any chosen orientation: YZ, XZ or XY.
When a piece orientation has been chosen, there are several ways to motility between slices. You can elevate the slider handle, utilize the up/down pointer keys or enter the slice number in the numerical field.
Before starting with the get-go profile line, switch the pointer from the Navigate to Select mode and adjust the position of the drawing aeroplane. In the profile line creation method, there are 2 different modes: Agile and Inactive. The main function of the Active mode is to create a contour line. In the Inactive mode, the existing contour lines can be modified or deleted. You tin select the respective mode by using the Describe button
The pointer's appearance on the screen depends on the selected drawing mode. The symbol on the screen changes from an arrow in the Inactive mode to a cross in the Agile mode.
Under the Mode tab, several drawing mode tools are provided, allowing yous to select areas in the current slice for contour line drawing Click on a tool icon activates a drawing tool.
Click: Clicking inside the epitome inserts a point, vertices. Successive vertices are connected with a straight-line segment.
Time: Click one time on the contour and then move the mouse forth the contour. The number of vertices depends on the fourth dimension interval (ms), specified in the corresponding field.
Distance: Click once on the contour and then just movement the mouse along the contour. The number of vertices depends on the specified distance interval. This cartoon style is recommended if the construction is shine.
Circle: The Circle tool e'er inserts a round contour line. The cursor position indicates the centre of the contour line. The number of contour vertices to be inserted and the circle radius depend on the specified values. Alternatively, you can use the mouse wheel to alter the circle radius.
Isoline: The Isoline cartoon mode detects the compatible intensity value under the mouse pointer and creates the largest connected area that contains all iso-value points. The current intensity value is displayed in the parameter field.
Magic wand: The Magic wand tool creates a profile line by selecting the largest connected surface area that contains the maximum intensity voxels and all voxels within a user-defined tolerance range. The Tolerance range defines the tool'southward sensitivity; the higher value yous set then wider image area that is selected.
You tin draw as many contours on a plane as required. Repeat the process until the contours are created for all slices. Under the Board tab, you lot can adjust the Visibility of the previously fatigued profile lines.
When you are satisfied with the created contour lines positions, click on the Create Surface button
to create a surface between the previously drawn contours.
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Source: https://imaris.oxinst.com/learning/view/article/contour-surface
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